Characterization of sulfate transport in desulfovibrio. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Bioreduction and biocrystallization of palladium by desulfovibrio. Sulfate reducing bacteria srb 1 are a broad group of bacteria that have in common the fact that they derive energy from the respiration of sulfate.
Accumulation was not influenced by incubation in nacl instead of kcl, by acidic ph 5. It is generally found in soils, waters, and the stools of animals, although in rare cases it has been found to cause infection in humans. Effects of oxygen on the growth of desulfovibrio desulfuricans. The sulfate reducing bacterium desulfovibrio desulfuricans inhabits both. Desulfovibrio vulgaris, desulfovibrio desulfuricans, and desulfotomaculum. Sulfate reducing bacteria are responsible for significant corrosion of equipment. Although error bars resulting from combining data from independent experiments designed in. The toxicity of al to desulfovibrio desulfuricans g20 was assessed over a period of 8 weeks in a modi. Structural and biological lipid a characterization wei zhangsun equipe endotoxins.
Desulfovibrio desulfuricans isolates from the gut of a. Extracellular ironsulfur precipitates from growth of. Pdf the treatment of wellwater containing bacteria desulfovibrio desulfuricans used. Nitrate reduction was induced by nitrate or limiting concentrations of sulfate during growth. Complete genome sequence of desulfovibrio desulfuricans. Desulfovibrio article about desulfovibrio by the free. Here, we report the draft genome of the gramnegative, sulfatereducing bacterium desulfovibrio desulfuricans strain g11. Desulfovibrio desulfuricans atcc 541 was obtained from the american type. Sulfate reducing bacteria problems in water treatment. Desulfomonas pigra atcc 29098t, desulfovibrio desulfuricans subsp. Under continuous culture conditions two effects of. Threedimensional structure of the nonaheme cytochrome c from. The sulfatereducing bacterium desulfovibrio desulfuricans strain csn dsm 104 oxidized h 2 with thiosulfate, sulfate, sulfite, nitrite, nitrate and oxygen with rates increasing in the order listed from 20 to 525 nmol h 2 min. Recently, different studies demonstrated that lipopolysaccharides lps from gut bacteria and their.
A combined pathway of sulfur compound disproportionation in. Reduction of the oxidized metal species resulted in the precipitation of solid phases of the metals. Dde i from desulfovibrio desulfuricans, strain norway sigma. Its cells are gramnegative, slightly curved rods gilmour et al. Pdf biogasenergy is marginally profitable against the parasitic energy demands of processing biomass. The worlds first wiki where authorship really matters nature genetics, 2008. Under aerobic conditions at 37c with 5% co2, no growth was obtained.
Identification of these species with conventional methods is difficult. Isolated from a rumen fluid enrichment, this culture has been a model syntrophic partner due to its metabolic flexibility. It has 907 residues on a single polypeptide chain, a. Desulfovibrio desulfuricans strain g20 is a spontaneously nalidixic acid resistant derivative of the wildtype strain g100a that was isolated from an oil well corrosion site weimer et al. Direct spectroscopic evidence for the presence of a 6fe cluster in an ironsulfur protein isolated from desulfovibrio desulfuricans atcc 27774 published erratum appears in j. Strain details desulfovibrio desulfuricans strain g20 is a spontaneously nalidixic acid resistant derivative of the wildtype strain g100a that was isolated from an oil well corrosion site weimer et al. Desulfovibrio desulfuricans desulfuricans beijerinck, 1895 kluyver and van niel, 1936.
Desulfovibrio desulfuricans is a gramnegative sulfatereducing bacteria. The quality of the spectra is similar, and two peaks were common to both spectra at mz 60. Therefore, manipulation and growth of cultures should be performed in an anaerobic growth chamber coy laboratory product, inc. Threedimensional structure of the nonaheme cytochromec from desulfovibrio desulfuricans essex in the feiii state at 1. Desulfovibrio longus desulfovibrio piger desulfovibrio longreachensis desulfovibrio oxamicus desulfovibrio multispirans. At ph 5, cell population densities decreased signi. Feio1, vitaly zinkevich1, enric llobetbrossa2, peter eaton1, jurgen schmitt3, jean guezennec4 7 and iwona b. Imbalances in the composition of gut microbiota have been reported to be associated with susceptibility to obesity and insulin resistance diseases 1, 2.
The primary and threedimensional structures of a ninehaem. Get a printable copy pdf file of the complete article 1. Under anoxic conditions, certain anaerobic bacteria can oxidize dissolved elemental mercury and convert the oxidized hg to neurotoxic methylmercury. By combining the uf with flocculation process, bacteria had been removed until. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers like other sulfatereducing bacteria, desulfovibrio was long considered to. Desulfovibrio desulfuricans was grown on a medium with an excess of pyruvate in relation to sulphate, so that the pyruvate could be dissimilated both by respiration and by fermentation. Imagine a global collaborative knowledge base for original thoughts. Growth of desulfovibrio desulfuricans with sulfate, nitrate, or nitrite as the only electron acceptor. Pdf crystallization and preliminary xray analysis of a. Johns hospital health center, santa monica, california 90404 3. Aminoacidsequence of the cytochromec3 mr26000 from desulfovibrio desulfuricans norway and a comparison with those of the other polyhemic cytochromes from desulfovibrio.
The authors have examined extracellular ironbearing precipitates resulting from the growth of desulfovibrio desulfuricans in a basal medium with lactate as the carbon source and ferrous sulfate. Biochimica et biophysica actaprotein structure and molecular enzymology 12051. Metalmining bacteria are green chemists sciencedaily. Under aerobic conditions at 37c with 5% co 2, no growth was obtained. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. The two most common species of these bacteria, known scientifically as desulfovibrio desulfuricans or desulfotomaculum nigrificans, can be the cause of severe pitting corrosion even on stainless steel components. Wall, university of missouri gerrit voordouw, university of calgary. The role of rnf in ion gradient formation in desulfovibrio. Desulfovibrio africanus desulfovibrio desulfuricans desulfovibrio fructosovorans desulfovibrio gigas desulfovibrio salexigens desulfovibrio vulgaris desulfovibrio sp. In this study, we conducted experiments with the hgmethylating bacterium desulfovibrio desulfuricans. While most isolates have not been identified to the species level, desulfovibrio fairfieldensis and d. Desulfovibrio desulfuricans beijerinck, 1895 kluyver and van niel, 1936.
Atp sulfurylase from desulfovibrio desulfuricans atcc 27774 native. This name appeared on the approved lists of bacterial names 1980. The two negativeion mass spectra gave a rough idea of the degree of heterogeneity present in the preparation. Since these bacteria are killed by exposure to atmospheric oxygen, the environmental niches most frequently occupied. Kroneck fachbereich biologie, mathematischnaturwissenschaftliche sektion, universita. Chemolithotrophic growth of desulfovibrio desulfuricans. Isolation and characterization of desulfovibrio growing on. According to a previous study 5 we expected that disproportionation is catalyzed by thi osulfate reductase and some of the enzymes involved in sulfate. Desulfovibrio choose one desulfovibrio desulfuricans strain atcc 27774 dsm 6949 desulfovibrio desulfuricans dsm 642 desulfovibrio desulfuricans nd2 all lower taxonomy nodes 3 common name i. Desulfovibrio desulfuricans strain 27774 is one of a relative small group of. Both positive anaerobic blood cultures were further subcultured on columbia blood agar with 5% horse blood and on chocolate agar. The sulfatereducing bacterium aldehyde oxidoreductase from desulfovibrio gigas mop is a member of the xanthine oxidase family of enzymes.
Request pdf bioreduction and biocrystallization of palladium by desulfovibrio. Desulfovibrio cells have mesophillic growth patterns with an ideal growth rate between the temperatures of twentyfive to fourty degrees celcius ncbi. The ninehaem cytochrome c 9hcc, previously described as having 12 haem groups, was isolated from cells of desulfovibrio desulfuricans atcc 27774, grown under both nitrate and sulphaterespiring conditions. Pdf upconversion of cellulosic waste into a potential drop in. Desulfovibrio vulgaris an overview sciencedirect topics. The product of this respiration, sulfide, is highly toxic and corrosive and is responsible for the environmental, economic, and health impact of these bacteria in their habitats. Desulfovibrio desulfuricans strain g20, which was found at an oil well corrosion site 2can. Moreover, pdnaps could be synthesized by desulfovibrio desulfuricans. M or nmolmg protein to cell suspensions incubated in 150 mm kcl at1c were almost completely taken up and accumulated about 5,000fold. The rcsb pdb also provides a variety of tools and resources.
Desulfovibrio strains have been found in a variety of habitats, including soil, the intestines and feces of animals, and both salinic and fresh water. All lower taxonomy nodes 3 common name i synonym iother names i bacillus desulfuricans beijerinck 1895 saltet 1900 microspira desulfuricans beijerinck 1895 migula. We propose the use of desulfovibrio desulfuricans nd2 as a model species for understanding the mechanism of microbial hg. Desulfovibrio desulfuricans atcc 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. Dde i from desulfovibrio desulfuricans, strain norway. The primary and threedimensional structures of a nine. Jan 15, 2020 525146 desulfovibrio desulfuricans strain atcc 27774 dsm 6949 strain.
It is particularly noted for its ability to produce methyl mercury. Sulfate respiration in desulfovibrio vulgaris hildenborough. The subspecies desulfovibrio desulfuricans desulfuricans was originally described by kluyver and van niel 1936. By combining in vitro experiments with bioinformatic and rnaseq. Desulfovibrio desulfuricans aestuarii postgate and campbell, 1966 subspecies.
Desulfovibrio desulfuricans strain atcc 27774 dsm 6949. Desulfovibrio desulfuricans isolates from the gut of a single. Sulfatereducing bacterium desulfovibrio desulfuricans. Lactate or h2 served as the electron donor and the oxidized crvi, movi, sevi and uvi served as electron acceptors. Dde i from desulfovibrio desulfuricans strain norway.
Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers. Degradation of nitrocellulosebased paint by desulfovibrio. Sulfatereducing bacterium desulfovibrio desulfuricans nd2 as a model for understanding bacterial mercury methylation article pdf available in applied and environmental microbiology 7712. A case of liver abscess with desulfovibrio desulfuricans. Desulfovibrio is known as a sulfate reducing bacterium, which has put it to the forefront of biological research. The disposal of elemental mercury hg0 wastes in mining and manufacturing areas has caused serious soil and groundwater contamination issues. The yield per mole fermented pyruvate is much lower than the yield per mole respired pyruvate. Search thousands of articles and collaborate with scientists around the globe. Desulfovibrio desulfuricans bacteremia in an immunocompromised. However, under anaerobic conditions, small colonies of motile bacilli were observed 48 h after inoculation. Desulfovibrio desulfuricans also known as strain nd 2 description and significance. Sulfatereducing bacterium desulfovibrio desulfuricans nd2 as. Alden research laboratory, 1 infectious diseases division, 2 microbiology laboratory, st. Discussion desulfovibrio desulfuricans has a constitutive thio sulfate reductase like most sulfatereducing bacteria and a constitutive capacity of thiosulfate dispropor tionation 5.
Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells. Desulfovibrio desulfuricans strain nd 2 was isolated from chesapeake bay sediments in may 1985 gilmour et al. Desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell il6 and il8 secretion and eselectin and vcam1 expression. The reported case of a desulfovibrio desulfuricans bacteremia occurring in an immunocompromised host with ulcerative colitis confirms that this organism may be a possible opportunistic human. Most desulfovibrio can tolerate small amounts of exposure to air. Reclassification of the only species of the genus desulfomonas. Cappitelli a a dipartimento di scienze e tecnologie alimentari e microbiologiche, universita degli studi di milano, via celoria 2, 203 milan, italy.
Desulfovibrio desulfuricans beijerinck 1895 kluyver and van niel 1936, 397al spirillum desulfuricans beijerinck 1895, 1. Negativeion maldi mass spectra of intact srb 1 and srb 2 lipid a, respectively, are presented on fig. Beech1 8 9 1 school of pharmacy and biomedical sciences, university of portsmouth, st. Pdf structure refinement of the aldehyde oxidoreductase. The reported case of a desulfovibrio desulfuricans. Degradation of nitrocellulosebased paint by desulfovibrio desulfuricans atcc 541 l.
Corrosion of iron and formation of iron phosphide by. Barrett and sinclair, 1967 is autoxidizable but does not combine with cn or co. Desulfovibrio desulfuricans isolates from the gut of a single individual. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. Despite recent progress, the specific factors that cause or ameliorate these diseases largely remain to be identified. Desulfovibrio desulfuricans survived exposure to air on the surface of agar plates for at least 48 h. Pdf sulfatereducing bacterium desulfovibrio desulfuricans. Haemcontaining proteins are directly involved in electron transfer as well as in enzymatic functions.
Eight of the 16 isolates 50% have been obtained from patients with appendicitis andor intraabdominal abscesses, and in six other cases. Desulfovibrio is a genus of gramnegative sulfatereducing bacteria. Dna substrate, results in 100% cutting, 95% of fragments can be ligated, and 95% recut. Intracellular hg0 oxidation in desulfovibrio desulfuricans.
Threedimensional structure of the nonaheme cytochrome c. Gramnegative sulphatereducing bacterium desulfovibrio desulfuricans 29 30 31. Models for the primary and threedimensional structures of this. Preferential reduction of the thermodynamically less favorable. Because of its metal corroding ability, which has consequently led to numerous health and safety concerns in industry, a means to neutralize and better understand desulfovibrio species is the goal of research on desulfovibrio itqb. Reduction of uranium by desulfovibrio desulfuricans.
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